RNA from mammalian cells: Affymetrix recommends using Qiagen's RNeasy Total RNA isolation kit.
RNA from mammalian tissue: Affymetrix recommends isolating total RNA with a commercial reagent such as TRIzol. All samples MUST be cleaned up using the Qiagen RNeasy Mini Kit before turning them into this facility.
RNA quality should be checked before turning the RNA into us. This includes at least looking at the 260/280 ratio and running the samples out on an agarose gel to check for completely intact RNA. It is highly recommended checking the quality of your sample using the Agilent Bioanalyzer if possible.
A minimum of 14 µg* of total RNA at a minimum concentration of 0.6 µg/µl is required for each sample that is turned in. The chips to be used for your experiment are to be turned in with the samples also. The chips are purchased through Affymetrix. You will receive the academic pricing for the chips from Affymetrix.
*We are transitioning to a new protocol where a minimum of 2 µg* of total RNA at a minimum concentration of 100 ng/µl is required for each sample that is turned in.